mastercycler gradient 5331 thermal cycler (Eppendorf AG)

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Eppendorf AG mastercycler gradient 5331 thermal cycler
Mastercycler Gradient 5331 Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5331+mastercycler+gradient+thermal+cycler/Eppendorf/pm41571226-90-8-13
Average 99 stars, based on 201118 article reviews

mastercycler gradient 5331 thermal cycler - by Bioz Stars, 2026-10

99/100 stars

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Polymerase Chain Reaction:

Article Title: Genotype-phenotype analyses of Iranian patients with hemophilia B (Leyden -) and hemophilia B (Leyden +): A single-center study.
Article Snippet: Background: There is a high prevalence of inherited bleeding disorders in Iran, such as hemophilia A (HA) and hemophilia B (HB).. This study aimed to analyze the molecular and clinical profiles of patients with HB.. Methods: A single-center study was conducted among patients with severe HB between March 20, 2000, and June 31, 2023.

Article Title: Experimental Validation of miR-4443, miR-572, and miR-150-5p in Serum and Tissue of Breast Cancer Patients as a Potential Diagnostic Biomarker: A Study Based on Bioinformatics Prediction.
Article Snippet: Breast cancer is the most common invasive cancer diagnosed in females and is also the main cause of cancer-related deaths leading to more than 500,000 deaths annually.. The present study aims to identify a promising panel of microRNAs (miRNAs) using bioinformatics analysis, and to clinically validate their utility for diagnosing breast cancer patients with high accuracy in a clinical setting.. First, in the in silico phase of our study, using bioinformatics analysis and the data available in the GEO database, miRNAs that were increased in the interstitial fluid of the tumor tissues (differentially expressed miRNAs), were screened and their related target genes were selected.

Article Title: Draft whole genome sequence of Paenibacillus sp., a novel facultative anaerobic bacteria isolated from an inflammatory bowel disease patient
Article Snippet: .. For PCR amplification, extracted DNA was run in an Eppendorf 5331 Mastercycler Gradient Thermal Cycler (Eppendorf, Germany) with the following settings: denaturation process for two minutes as 94°C, annealing process for 30 cycles (one minute at 94 °C, 45 s at 55 °C, and one minute at 72 °C), and elongation process for ten minutes at 72 °C for 29 cycles. .. The PCR product obtained was outsourced to Apical Scientific Sdn.

Article Title: Circulating microbiome DNA in Southern Ocean seabirds: A novel tool for disease surveillance in polar ecosystems
Article Snippet: .. All PCR reactions were conducted in an Eppendorf 5331 MasterCycler Gradient Thermal Cycler (Eppendorf, Hamburg, Germany) with a total reaction volume of 25 μL. .. Each reaction contained 1 × standard Taq buffer (10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, pH 8.3), 0.4 μM of each primer, 2.5 mM MgCl2, 0.4 mM dNTPs, 0.65 U Taq DNA polymerase (NEB, Mississauga, ON, Canada), and 1–50 ng of genomic DNA as the template.

Article Title: A novel variant in MFN2 linked to a lethal disorder of neonatal onset
Article Snippet: The Takara LA Taq Polymerase (Takara Bio, RR002M) was used with 250ng total cellular DNA. .. PCR cycling conditions were 94°C for 1 min; 98°C for 10 s and 68°C for 11 min (step 2 and 3- 35 cycles); and a final extension cycle at 72°C for 10 min, using an Eppendorf ® 5331 MasterCycler Gradient Thermal Cycler. ..

Amplification:

Reverse Transcription:

Article Title: The Influence of AQP5 on the Response to Hydrogen Peroxide in Breast Cancer Cell Lines
Article Snippet: RNA purity and concentration were assessed spectrophotometrically using the NanoPhotometer ® N60 (Implen GmbH, München, Germany). .. Subsequently, 1 μg of RNA was reverse-transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (4368814, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol on an Eppendorf 5331 MasterCycler Gradient Thermal Cycler (Eppendorf, Hamburg, Germany). ..

Article Title: The Influence of AQP5 on the Response to Hydrogen Peroxide in Breast Cancer Cell Lines
Article Snippet: RNA purity and concentration were assessed spectrophotometrically using the NanoPhotometer® N60 (Implen GmbH, München, Germany). .. Subsequently, 1 μg of RNA was reversetranscribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (4368814, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol on an Eppendorf 5331 MasterCycler Gradient Thermal Cycler (Eppendorf, Hamburg, Germany). ..

other:

Article Title: The role of matrix stiffness and viscosity on lipid phenotype and fat lineage potential
Article Snippet: Autologous fat transfer is a common procedure that patients undergo to rejuvenate large soft tissue defects.. However, these surgeries are complicated by limited tissue sources, donor-site morbidity, and necrosis.. While the biofabrication of fat tissue can serve as a clinical option for reconstructive surgery, the influence of matrix mechanics, specifically stiffness and viscosity, on adipogenesis requires further elucidation.

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